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Dodiya, Tanvi
- HPTLC Method Development and Validation for Simultaneous Estimation of Berberine, Ellagic Acid and Ferulic Acid in Amrtadi Churna
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Authors
Affiliations
1 Parul Institute of Pharmacy and Research, Parul University, Limda, Waghodia, Vadodara - 391760, Gujarat, IN
1 Parul Institute of Pharmacy and Research, Parul University, Limda, Waghodia, Vadodara - 391760, Gujarat, IN
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Journal of Natural Remedies, Vol 22, No 4 (2022), Pagination: 649-657Abstract
The objective of the current study was to develop a new simple and precise High Performance Thin Layer Chromatography (HPTLC) method for standardization of three biomarkers i.e., berberine, ellagic acid and ferulic acid in Amrtadi churna. Amrtadi churna is mainly used for hyperacidity and contains dried plant parts of Gokshur, Amla and Guduchi. The method was developed and validated using precoated silica gel at 60 F254 as the stationary phase and toluene:ethyl acetate:formic acid:methanol (6:6:1.6:0.4, v/v/v/v) as the mobile phase. The detection and quantification were performed at 319 nm and the Rf value obtained was 0.35±2 for berberine, 0.5±2 for ellagic acid and 0.74±2 for ferulic acid. The method was validated as per ICH guidelines in terms of linearity, precision, specificity, accuracy and robustness.Keywords
Amla, Gokshur, Guduchi, ICH, StandardizationReferences
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- Vaidya V, Kondalkar P, Shinde M, Gotmare S. HPTLC fingerprinting for simultaneous quantification of harmine, kaempferol, diosgenin and oleic acid in the fruit extract of Tribulus terrestris and its formulation. Int. J. Pharm. Sci. Res. 2018; 9:3066-74.
- Balekundri A, Mannur V. Quality control of the traditional herbs and herbal products: a review. Future Journal of Pharmaceutical Sciences. 2020; 6(1):1-9. https://doi.org/10.1186/s43094-020-00091-5 DOI: https://doi.org/10.1186/s43094-020-00091-5
- Sherma J. Review of HPTLC in drug analysis: 1996-2009. Journal of AOAC international. 2010; 93(3):754-64. https://doi.org/10.1093/jaoac/93.3.754 DOI: https://doi.org/10.1093/jaoac/93.3.754
- Sonia K, Lakshmi KS. HPTLC method development and validation: An overview. Journal of Pharmaceutical Sciences and Research. 2017; 9(5):652.
- Shivatare RS, Nagore DH, Nipanikar SU. HPTLC’an important tool in standardization of herbal medical product: A review. J Sci Innov Res. 2013; 2(6):1086-96.
- Namjoshi, A.M. Ayurvedic formulary of India. Controller of publications, Delhi. 1976.
- Maithani AL, Parcha VE, Kumar DE. Quantitative estimation of berberine content of Berberis asiatica from different altitude of Garhwal Himalaya. Asian J Pharm Clin Res. 2014; 7(1):165-7.
- Prajwala B, Raghu N, Gopenath TS, Shanmukhappa B, Karthikeyan M, Ashok G, Ranjith MS, Srinivasan V, Kanthesh MB. Berberine and its pharmacology potential: A review. Eur J Biomed. 2020; 7:115-23.
- Goswami AK, Gogoi N, Shakya A, Sharma HK. Development and validation of high-performance thin-layer chromatographic method for quantification of berberine in rhizomes of Coptis teeta Wall, an endangered species collected from Arunachal Pradesh, India. Journal of chromatographic science. 2019; 57(5):411-7. https://doi.org/10.1093/chromsci/ bmz009 DOI: https://doi.org/10.1093/chromsci/bmz009
- Cai Z, Wang C, Yang W. Role of berberine in Alzheimer’s disease. Neuropsychiatric disease and treatment. 2016; 12:2509. https://doi.org/10.2147/ NDT.S114846 14. Khan KH. Roles of Emblica officinalis in medicine-A review. Bot Res Int. 2009; 2(4):218-28.
- Dasaroju S, Gottumukkala KM. Current trends in the research of Emblica officinalis (Amla): A pharmacological perspective. Int J Pharm Sci Rev Res. 2014; 24(2):150-9.
- Veni KN, Meyyanathan SN, Suresh B. Analysis of Ellagic Acid in Fresh and processed fruit products by High Performance Thin Layer Chromatography. International Research Journal of Pharmacy. 2012; 3(7):201-204.
- Akram M, Asif HM, Akhtar N, Shah PA, Uzair MU, Shaheen G, Shamim T, Shah SA, Ahmad K. Tribulus terrestris Linn.: a review article. J Med Plants Res. 2011; 5(16):3601-5.
- Boz H. Ferulic acid in cereals-a review. Czech Journal of Food Sciences. 2015; 33(1):1-7. https://doi.org/10.17221/401/2014-CJFS DOI: https://doi.org/10.17221/401/2014-CJFS
- Chhatre S, Nesari T, Somani G, Kanchan D, Sathaye S. Phytopharmacological overview of Tribulus terrestris. Pharmacognosy reviews. 2014; 8(15):45. https://doi.org/10.4103/0973-7847.125530 DOI: https://doi.org/10.4103/0973-7847.125530
- Hingse SS, Digole SB, Annapure US. Method development for simultaneous detection of ferulic acid and vanillin using high-performance thin layer chromatography. Journal of Analytical Science and Technology. 2014; 5(1):1-9. https://doi.org/10.1186/ s40543-014-0021-6 DOI: https://doi.org/10.1186/s40543-014-0021-6
- International Conference on Harmonization, ICH Q2 (R1): Validation of Analytical Procedures: Text and Methodology, ICHSecretariat, Geneva. 2005.
- Extraction, Isolation and Structure Elucidation of a Bioactive Constituent from Chloroform Extract of Moringa concanensis Leaves
Abstract Views :90 |
PDF Views:100
Authors
Affiliations
1 Department of Quality Assurance, Faculty of Pharmacy, Parul University, Vadodara, Gujarat, IN
2 Department of Pharmacognosy, Faculty of Pharmacy, Parul Institute of Pharmacy & Research, Parul University, Vadodara, Gujarat, IN
1 Department of Quality Assurance, Faculty of Pharmacy, Parul University, Vadodara, Gujarat, IN
2 Department of Pharmacognosy, Faculty of Pharmacy, Parul Institute of Pharmacy & Research, Parul University, Vadodara, Gujarat, IN
Source
Journal of Natural Remedies, Vol 22, No 4 (2022), Pagination: 673-682Abstract
Moringa concanensis Nimmo. (Moringaceae) is a wild plant found in India. Traditionally, tribes used this plant as an antioxidant, anticancer, anticonvulsant, antimicrobial, antifertility, anti-inflammatory, antipyretic, and analgesic. In the present study, the leaves of Moringa concanensis were subjected to successive solvent extraction with five different solvents of increasing polarity, i.e., petroleum ether, chloroform, ethyl acetate, methanol, and water, followed by a phytochemical screening of each extract. The phytochemical study indicated the presence of terpenoids, steroids, cardiac glycosides, flavonoids, alkaloids, carbohydrates, proteins, tannins, and phenolics. The chloroform extract showed the presence of cardiac glycosides (cardenolides); therefore, a RP-HPLC method was developed to identify the number of constituents in the extract. The extract was recrystallized to isolate the single component and to gain the desired purity for further spectral analysis. Different spectral data from UV-Visible, Infrared, Mass, and Nuclear Magnetic Resonance spectroscopy were analyzed and the isolated chemical constituent was identified.Keywords
Cardiac Glycosides, Infrared Spectroscopy, Mass Spectroscopy, RP-HPLC, Structure ElucidationReferences
- Abd Rani NZ, Husain K, Kumolosasi E. Moringa genus: a review of phytochemistry and pharmacology. Frontiers in Pharmacology. 2018; 9:108. https://doi.org/10.3389/ fphar.2018.00108 DOI: https://doi.org/10.3389/fphar.2018.00108
- Flowersofindia.net. 2022. Moringa concanensis - Konkan Moringa. [online] Available at: https://flowersofindia.net/ catalog/slides/Konkan%20Moringa.html
- India Biodiversity Portal. 2022. Moringa concanensis Nimmo. | Species. [online] Available at: https://indiabiodiversity. org/species/show/230449
- Vijayarajan M, Pandian RM. Antioxidant and free radical scavenging activity of Moringa concanensis root bark extract. International Journal of Zoology and Applied Biosciences. 2016; 1:46-56.
- Ankita D, Sauryya B, Kumar PT. Research in Chemistry and Environment.
- Balamurugan V, Balakrishnan V, Robinson JP, Ramakrishnan M. Anti-cancer and apoptosis-inducing effects of Moringa concanensis using hepG2 cell lines. Bangladesh Journal of Pharmacology. 2014; 9(4):604-09. https://doi.org/10.3329/ bjp.v9i4.20481 DOI: https://doi.org/10.3329/bjp.v9i4.20481
- Vijayakumar S, Bhuvaneshwari V, Sumathi A. Antioxidant and anticancer potential of methanolic leaf extract of Moringa concanensis Nimmo. against human breast cancer cell line MCF-7. International Journal of Pharmacognosy and Phytochemical Research. 2017; 9(6):750-4. https://doi. org/10.25258/phyto.v9i6.8172 DOI: https://doi.org/10.25258/phyto.v9i6.8172
- Joy AE, Kunhikatta SB, Manikkoth S. Anti-convulsant activity of ethanolic extract of Moringa concanensis leaves in Swiss albino mice. Archives of Medicine and Health sciences. 2013; 1(1):6. https://doi.org/10.4103/2321- 4848.113548 DOI: https://doi.org/10.4103/2321-4848.113548
- Karmegam N, Nagaraj R. Combinatorial antibacterial activity of selective medicinal plant species from Southern Eastern Ghats. In National Seminar on Recent Advances in Biotechnology and Applied Microbiology (Salem). 2017.
- Balamurugan V, Balakrishnan V. Evaluation of phytochemical, Pharmacognostical and antimicrobial activity from the bark of Moringa concanensis Nimmo. Int J Curr Microbiol App Sci. 2013; 2:117-25.
- Ravichandiran V, Suresh B, Sathishkumar MN, Elango K, Srinivasan R. Antifertility activity of hydro alcoholic extract of Moringa concanensis Nimmo.: An ethnomedicines used by tribals of Nilgiris region in Tamilnadu. Advances in Traditional Medicine. 2007; 7(2):114-20. https://doi. org/10.3742/OPEM.2007.7.2.114 DOI: https://doi.org/10.3742/OPEM.2007.7.2.114
- Singh A, Mishra JN, Singh SK. Pharmacological importance of Moringa concanensis Nimmo. leaf: An overview. Asian Journal of Pharmaceutical and Clinical Research. 2019; 27-31. https://doi.org/10.22159/ajpcr.2019.v12i2.28979 DOI: https://doi.org/10.22159/ajpcr.2019.v12i2.28979
- Jayabharathi M, Chitra M. Evaluation of anti-inflammatory, analgesic and antipyretic activity of Moringa concanensis Nimmo. J Chem Pharm Res. 2011; 3(2):802-6.
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- Bakoriya R, Soni KK, Thomas T. Isolation and Structural Elucidation of Bioactive Compound from Ethanolic Extract of Cassia tora Leaves. Asian Journal of Chemistry. 2015; 27(10):3749-52. https://doi.org/10.14233/ ajchem.2015.18952 DOI: https://doi.org/10.14233/ajchem.2015.18952
- Khandelwal K. Practical pharmacognosy. Pragati Books Pvt. Ltd. 2008.
- Milenkovic MZ, Marinkovic VD, Sibinovic PS, Palic RM, Milenovic DM. An HPLC method for the determination of digoxin in dissolution samples. Journal of the Serbian Chemical Society. 2010; 75(11):1583-94. https://doi. org/10.2298/JSC100106123M DOI: https://doi.org/10.2298/JSC100106123M
- U.S. National Library of Medicine. (n.d.). Hazardous Substances Data Bank (HSDB): 214 - pubchem. National Center for Biotechnology Information. PubChem Compound Database. 2022.https://pubchem.ncbi.nlm.nih. gov/source/hsdb/214
- Development and Validation of HPTLC Method for Simultaneous Estimation of Piperine and Scopoletin in Ajmodadi churna
Abstract Views :105 |
PDF Views:73
Authors
Affiliations
1 Parul Institute of Pharmacy and Research, Parul University, Limda, Waghodia - 391760, Vadodara, Gujarat, IN
1 Parul Institute of Pharmacy and Research, Parul University, Limda, Waghodia - 391760, Vadodara, Gujarat, IN
Source
Journal of Natural Remedies, Vol 23, No 1 (2023), Pagination: 143-148Abstract
In this recent study, several trials were made to develop a HPTLC method for quantification of scopoletin and piperine in the Ajmodadi formulation. HPTLC was done on pre-coated silica gel 60 F254 plates with a mobile phase of toluene:ethyl acetate:methanol:formic acid (3.9:3.9:0.3:1.7 v/v/v/v). The retardation factors for scopoletin 0.75 and for piperine was 0.86 and found a good and defined resolution peak. Densitometer analysis of scopoletin and piperine was carried out at 335 wavelength (nm). The developed method was validated as reported in ICH guidelines (Q2R1). A linearity study shows that scopoletin and piperine was linear, and the recovery studies revealed a recovery in between 98-102 % as per guideline. This method was established to be rapid, delicate, accurate and specific, thus, these methods may be used in the quantification of the drug in any polyherbal formulation.Keywords
Ajmodadi Churna, HPTLC, Piperine, Scopoletin.References
- Parasuraman S, Thing GS, Dhanaraj SA. Polyherbal formulation: Concept of ayurveda. Pharmacogn Rev. 2014; 8:73. https://doi.org/10.4103/0973-7847.134229 DOI: https://doi.org/10.4103/0973-7847.134229
- Verma S, Singh SP. Current and future status of herbal medicines. Vet. World. 2008; 1:347. https:// doi.org/10.5455/vetworld.2008.347-350 DOI: https://doi.org/10.5455/vetworld.2008.347-350
- Hazra AK, Chakraborty B, Mitra A, Sur TK. A rapid HPTLC method to estimate piperine in Ayurvedic formulations containing plant ingredients of Piperaceae family. J Ayurveda Integr Med. 2019; 10:248-254. https://doi.org/10.1016/j. jaim.2017.07.006 DOI: https://doi.org/10.1016/j.jaim.2017.07.006
- Sriwastava NK, Shreedhara CS, Ram HA. Standardization of Ajmodadi churna, a polyherbal formulation. Pharmacogn Res. 2019; 2:98. https://doi. org/10.4103/0974-8490.62957 DOI: https://doi.org/10.4103/0974-8490.62957
- Namjoshi AM. Ayurvedic formulary of India. Controller of Publications. 1976. Delhi, 85, page no. 106.
- https://www.bimbima.com/ayurveda/ajmodadichurna-for-arthritis-knee-and-joint-pain/1728/. Accessed 2 March 2022
- https://pubchem.ncbi.nlm.nih.gov/compound/ Piperine. Accessed 2 March 2022
- https://pubchem.ncbi.nlm.nih.gov/compound/ Scopoletin. Accessed 2 March 2022
- Vyas J, Itankar P, Tauqeer M, Kelkar A, Agrawal M. Development of HPTLC method for estimation of piperine, guggulsterone E and Z in polyherbal formulation. Pharmacogn J. 2013; 5:259-64. https:// doi.org/10.1016/j.phcgj.2013.10.003 DOI: https://doi.org/10.1016/j.phcgj.2013.10.003
- Prabhu C, Subramanian GS, Karthik A, Kini S, Rajan MS, Udupa. Determination of telmisartan by HPTLC–a stability indicating assay. JPC- J Planar Chromat.2007; 20:477-81. https://doi.org/10.1556/ JPC.20.2007.6.14 DOI: https://doi.org/10.1556/JPC.20.2007.6.14
- Patra KC, Kumar KJ. A validated HPTLC method for simultaneous analysis of eugenol and piperine in a siddha formulation. JPC–J Planar Chromat. 2010; 23:293-7. https://doi.org/10.1556/JPC.23.2010.4.11 DOI: https://doi.org/10.1556/JPC.23.2010.4.11
- ICH Q2R1 Validation of analytical procedures: methodology. International conference on harmonisation of technical requirements for registration of pharmaceuticals for human use (Q2R1).
- Estimation of Digoxin in Chloroform Extract of Moringa concanensis Leaves Using Newly Developed and Validated RP-HPLC Method
Abstract Views :111 |
PDF Views:81
Authors
Affiliations
1 Department of Quality Assurance, Faculty of Pharmacy, Parul University, Vadodara - 391760, Gujarat, IN
2 Parul Institute of Pharmacy and Research, Faculty of Pharmacy, Parul University, Vadodara - 391760, Gujarat, IN
1 Department of Quality Assurance, Faculty of Pharmacy, Parul University, Vadodara - 391760, Gujarat, IN
2 Parul Institute of Pharmacy and Research, Faculty of Pharmacy, Parul University, Vadodara - 391760, Gujarat, IN
Source
Journal of Natural Remedies, Vol 23, No 1 (2023), Pagination: 149-155Abstract
Cardiac glycosides are the secondary metabolites that are used in atrial fibrillation and atrial flutter as it binds with the sodium-potassium ATPase pump and increases the force of contraction. There are two types of cardiac glycosides i.e. cardenolides and bufadienolides. Digoxin is a cardenolide type of cardiac glycoside. Digoxin is found in chloroform extract of Moringa concanensis leaves. In the present study, the reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated to determine the content of digoxin in chloroform extract of M. concanensis leaves from three different geographical sources. The chromatographic method was developed at 220 nm wavelength with an Acetonitrile: Water: 1% Orthophosphoric acid (50:50:0.1 %V/V/V) mobile phase. In this method, the digoxin shows linearity in the range of 10 – 50 μg/ml with a regression coefficient of 0.995. The Limit of detection (LOD) and Limit of quantitation (LOQ) was found to be 0.232 μg/ml and 0.703 μg/ml respectively.Keywords
Chloroform Extract, Moringa concanensis, RP-HPLC Method, Soxhlet Extraction.References
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